Nano Archive

Synthesis of a novel biodegradable poly(ester amine) (PEAs) copolymer based on low-molecular-weight polyethyleneimine for gene delivery

Guo, QingFa and Shi, Shuai and Wang, XiuHong and Kan, Bing and Gu, YingChun and Shi, XingYu and Luo, Feng and Zhao, Xia and Wei, YuQuan and Qian, ZhiYong (2009) Synthesis of a novel biodegradable poly(ester amine) (PEAs) copolymer based on low-molecular-weight polyethyleneimine for gene delivery. International Journal of Pharmaceutics, In Press, Uncorrected Proof . - . (In Press)

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Official URL: http://www.sciencedirect.com/science/article/B6T7W...

Abstract

In this paper, a novel biodegradable poly(ester amine) (PEA) copolymer was successfully prepared from low-molecular-weight polyethyleneimine (PEI, Mn = 1800) and poly(-caprolactone)–Pluronic–poly(-caprolactone) (PCFC) copolymers. According to the results of agarose gel electrophoresis, particle sizes and zeta potential measurement and transfection efficiency, these PEA copolymers showed great ability to condense plasmid DNA effectively into nano-complexes with small particle size (≤200 nm) and moderate zeta potential (≥12 mV) at proper polymeric carrier/DNA weight ratio. Compared with low-molecular-PEI (Mn = 1800), the obtained PEAs exhibited higher transfection efficiency as well as lower cytotoxicity. These results indicated that such PEAs might have great potential application in gene delivery system.

Item Type:Article
Uncontrolled Keywords:Polyethyleneimine; Poly(-caprolactone)–Pluronic–poly(-caprolactone) copolymer; Poly(ester amine); Gene delivery; Cytotoxicity
Subjects:Biomedical Science > Nanobiotechnology
Material Science > Nanostructured materials
ID Code:5653
Deposited By:SPI
Deposited On:05 Aug 2009 10:12
Last Modified:05 Aug 2009 10:12

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