Nano Archive

A pH-sensitive fusogenic peptide facilitates endosomal escape and greatly enhances the gene silencing of siRNA-containing nanoparticles in vitro and in vivo

Hatakeyama, Hiroto and Itho, Erika and Akita, Hidetaka and Oishi, Motoi and Nagasaki, Yukio and Futaki, Shiroh and Harashima, Hideyoshi (2009) A pH-sensitive fusogenic peptide facilitates endosomal escape and greatly enhances the gene silencing of siRNA-containing nanoparticles in vitro and in vivo. Journal of Controlled Release, In Press, Uncorrected Proof . - . (In Press)

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Official URL: http://www.sciencedirect.com/science/article/B6T3D...

Abstract

Previously, we developed a multifunctional envelope-type nano device (MEND) for efficient delivery of both pDNA and siRNA. Modification of a MEND with polyuethylene glycol, i.e., PEGylation, is a potential strategy for in vivo delivery of MENDs to tumor tissue. However, PEGylation also inhibits both uptake and endosomal escape of MENDs. To overcome these limitations, we developed a PEG-peptide-DOPE (PPD) that can be cleaved in a matrix metalloproteinase (MMP)-rich environment. In this study, to further improve the silencing activity of encapsulated siRNA, we modified the PPD-MEND with a pH-sensitive fusogenic GALA peptide (GALA/PPD-MEND). First, we determined the GALA and PPD content that would optimize the synergistic functions of GALA and PPD. The most efficient gene silencing activity was achieved when GALA and either conventional PEG-lipid or PPD were used to modify the MEND at a molar ratio of 1:1. In this case, the silencing activity was comparable to that achieved when using a MEND that had not been modified with PEG (unmodified MEND). Furthermore, in vivo topical administration revealed that optimized PPD/GALA-MENDa resulted in more efficient gene silencing compared with unmodified MENDs. Collectively, data demonstrate that introduction of both of a pH-sensitive fusogenic GALA peptide and PPD into the MEND facilitates nanoparticle endosomal escape, thereby enhancing the efficiency of siRNA delivery and gene silencing.

Item Type:Article
Uncontrolled Keywords:Multifunctional envelope-type nano device (MEND); siRNA delivery; Intracellular trafficking; PPD; GALA
Subjects:Physical Science > Nano objects
Biomedical Science > Nanomedicine
ID Code:5650
Deposited By:SPI
Deposited On:05 Aug 2009 10:18
Last Modified:05 Aug 2009 10:18

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