Nano Archive

Entrapment of enzymes and carbon nanotubes in biologically synthesized silica: Glucose oxidase-catalyzed direct electron transfer

Ivnitski, Dmitri and Artyushkova, Kateryna and Rincon, Rosalba A. and Atanassov, Plamen and Luckarift, Heather R. and Johnson, Glenn R. (2008) Entrapment of enzymes and carbon nanotubes in biologically synthesized silica: Glucose oxidase-catalyzed direct electron transfer. SMALL, 4 (3). pp. 357-364.

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Official URL: http://www3.interscience.wiley.com/journal/1179117...

Abstract

This work demonstrates a new approach for building bioinorganic interfaces by integrating biologically derived silica with single-walled carbon nanotubes to create a conductive matrix for immobilization of enzymes. Such a strategy not only allows simple integration into biodevices but presents an opportunity to intimately interface an enzyme and manifest direct electron transfer features. Biologically synthesized silica/carbon nanotube/enzyme composites are evaluated electrochemically and characterized by means of X-ray photoelectron spectroscopy. Voltammetry of the composites displayed stable oxidation and reduction peaks at an optimal potential close to that of the FAD/FADH(2) cofactor of immobilized glucose oxidase. The immobilized enzyme is stable for a period of one month and retains catalytic activity for the oxidation of glucose. It is demonstrated that the resulting composite can be successfully integrated into functional bioelectrodes for biosensor and biofuel cell applications.

Item Type:Article
Uncontrolled Keywords:electron transfer; glucose oxidase; nanocomposites; photoelectron spectroscopy; silica immobilization
Subjects:Material Science > Functional and hybrid materials
Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
ID Code:552
Deposited By:IoN
Deposited On:20 Jan 2009 16:49
Last Modified:29 Jan 2009 14:28

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