Yu, Zeta Tak For and Kamei, Ken-ichiro and Takahashi, Hiroko and Shu, Chengyi Jenny and Wang, Xiaopu and He, George Wenfu and Silverman, Robert and Radu, Caius G. and Witte, Owen N. and Lee, Ki-Bum and Tseng, Hsian-Rong (2009) Integrated microfluidic devices for combinatorial cell-based assays. Biomedical Microdevices, 11 (3). pp. 547-555.
The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-mu Chip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed "on-chip" transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-mu Chip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.
|Subjects:||Engineering > Nanotechnology applications in mechanical engineering|
|Deposited On:||25 Aug 2010 07:59|
|Last Modified:||25 Aug 2010 07:59|
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