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Integrated microfluidic devices for combinatorial cell-based assays

Yu, Zeta Tak For and Kamei, Ken-ichiro and Takahashi, Hiroko and Shu, Chengyi Jenny and Wang, Xiaopu and He, George Wenfu and Silverman, Robert and Radu, Caius G. and Witte, Owen N. and Lee, Ki-Bum and Tseng, Hsian-Rong (2009) Integrated microfluidic devices for combinatorial cell-based assays. Biomedical Microdevices, 11 (3). pp. 547-555.



The development of miniaturized cell culture platforms for performing parallel cultures and combinatorial assays is important in cell biology from the single-cell level to the system level. In this paper we developed an integrated microfluidic cell-culture platform, Cell-microChip (Cell-mu Chip), for parallel analyses of the effects of microenvironmental cues (i.e., culture scaffolds) on different mammalian cells and their cellular responses to external stimuli. As a model study, we demonstrated the ability of culturing and assaying several mammalian cells, such as NIH 3T3 fibroblast, B16 melanoma and HeLa cell lines, in a parallel way. For functional assays, first we tested drug-induced apoptotic responses from different cell lines. As a second functional assay, we performed "on-chip" transfection of a reporter gene encoding an enhanced green fluorescent protein (EGFP) followed by live-cell imaging of transcriptional activation of cyclooxygenase 2 (Cox-2) expression. Collectively, our Cell-mu Chip approach demonstrated the capability to carry out parallel operations and the potential to further integrate advanced functions and applications in the broader space of combinatorial chemistry and biology.

Item Type:Article
Subjects:Engineering > Nanotechnology applications in mechanical engineering
ID Code:5109
Deposited By:CSMNT
Deposited On:25 Aug 2010 07:59
Last Modified:25 Aug 2010 07:59

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