Nano Archive

Deformability study of breast cancer cells using microfluidics

Hou, H. W. and Li, Q. S. and Lee, G. Y. H. and Kumar, A. P. and Ong, C. N. and Lim, C. T. (2009) Deformability study of breast cancer cells using microfluidics. Biomedical Microdevices, 11 (3). pp. 557-564.

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Abstract

Cell deformability is an important biomarker which can be used to distinguish between healthy and diseased cells. In this study, microfluidics is used to probe the biorheological behaviour of breast cancer cells in an attempt to develop a method to distinguish between non-malignant and malignant cells. A microfabricated fluidic channel design consisting of a straight channel and two reservoirs was used to study the biorheological behaviour of benign breast epithelial cells (MCF-10A) and non-metastatic tumor breast cells (MCF-7). Quantitative parameters such as entry time (time taken for the cell to squeeze into the microchannel) and transit velocity (speed of the cell flowing through the microchannel) were defined and measured from these studies. Our results demonstrated that a simple microfluidic device can be used to distinguish the difference in stiffness between benign and cancerous breast cells. This work lays the foundation for the development of potential microfluidic devices which can subsequently be used in the detection of cancer cells.

Item Type:Article
Subjects:Engineering > Nanotechnology applications in mechanical engineering
ID Code:5108
Deposited By:CSMNT
Deposited On:25 Aug 2010 08:02
Last Modified:25 Aug 2010 08:02

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