Nano Archive

Development and characterization of hyaluronic acid-anchored PLGA nanoparticulate carriers of doxorubicin

Yadav, Awesh Kumar and Mishra, Pradeep and Mishra, Anil K. and Mishra, Pushpa and Jain, Sanyog and Agrawal, Govind Prasad (2007) Development and characterization of hyaluronic acid-anchored PLGA nanoparticulate carriers of doxorubicin. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 3 (4). pp. 246-257.

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Official URL: http://www.nanomedjournal.com/article/S1549-9634(0...

Abstract

A novel hyaluronic acid-poly(ethylene glycol)-poly(lactide-co-glycolide) (HA-PEG-PLGA) copolymer was synthesized and characterized by infrared and nuclear magnetic resonance spectroscopy. The nanoparticles of doxorubicin (DOX)-loaded HA-PEG-PLGA were prepared and compared with monomethoxy(polyethylene glycol) (MPEG)-PLGA nanoparticles. Nanoparticles were prepared using drug-to-polymer ratios of 1:1 to 1:3. Drug-to-polymer ratio of 1:1 is considered the optimum formulation on the basis of low particle size and high entrapment efficiency. The optimized nanoparticles were characterized for morphology, particle size measurements, differential scanning calorimetry, x-ray diffractometer measurement, drug content, hemolytic toxicity, subacute toxicity, and in vitro DOX release. The in vitro DOX release study was performed at pH 7.4 using a dialysis membrane. HA-PEG-PLGA nanoparticles were able to sustain the release for up to 15 days. The tissue distribution studies were performed with DOX-loaded HA-PEG-PLGA and MPEG-PLGA nanoparticles after intravenous (W) injection in Ehrlich ascites tumor-bearing mice. The tissue distribution studies showed a higher concentration of DOX in the tumor as compared with MPEG-PLGA nanoparticles. The in vivo tumor inhibition study was also performed after IV injection of DOX-loaded HA-PEG-PLGA nanoparticles up to 15 days. DOX-loaded HA-PEG-PLGA nanoparticles were able to deliver a higher amount of DOX as compared with MPEG-PLGA nanoparticles. The DOX-loaded HA-PEG-PLGA nanoparticles reduced tumor volume significantly as compared with MPEG-PLGA nanoparticles. (c) 2007 Elsevier Inc. All rights reserved.

Item Type:Article
Uncontrolled Keywords:hyaluronic acid; nanoparticles; doxorubicin; tumor targeting
Subjects:Biomedical Science > Nanomedicine
ID Code:510
Deposited By:INVALID USER
Deposited On:04 Dec 2008 12:08
Last Modified:04 Dec 2008 12:08

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