Nano Archive

Characterization of a microfluidic magnetic bead separator for high-throughput applications

Bu, Minqiang and Christensen, Troels B. and Smistrup, Kristian and Wolff, Anders and Hansen, Mikkel F. (2008) Characterization of a microfluidic magnetic bead separator for high-throughput applications. Sensors and Actuators A: Physical, 145-146 . 430 - 436.

Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.

Official URL: http://www.sciencedirect.com/science/article/B6THG...

Abstract

We present a novel microfluidic magnetic bead separator for high-throughput applications. The system consists of an array of small Nd–Fe–B permanent magnets arranged into a checkerboard pattern with alternating magnetization directions and an array of integrated permalloy elements encapsulated in the bottom of a microfabricated separation chamber. The permanent magnets provide magnetic forces on millimeter-scale, whereas the integrated permalloy elements enhance local forces hundredfold on 10 μm scale near the bottom of separation chamber. The fabrication of the chip is based on a multilayer SU-8 process combined with permalloy electroplating. The bead capturing behavior in a 10 μL separation chamber was demonstrated using 250 nm Nanomag-D® plain silica beads. The capture efficiency of the system was quantified using vibration sample magnetometry. The experimental results show that magnetic beads can be captured with efficiencies of 91% and 54% at flow rates of 1 mL/min and 4 mL/min, corresponding to average linear fluid velocities of 1.7 cm/s and 6.7 cm/s, respectively. The integration of soft magnetic elements in the chip results in a slightly higher capturing efficiency. However, the distribution of captured beads is much more uniform in the system with soft magnetic elements than in that without soft magnetic elements. The results demonstrate that the magnetic bead separator is capable of high-throughput sample preparation, e.g., for cleaning inhibitors in DNA samples prior to PCR amplification.

Item Type:Article
Additional Information:Special Issue: Transducers/07 Eurosensors XXI, The 14th International Conference on Solid State Sensors, Actuators and Microsystems and the 21st European Conference on Solid-State Transducers, The 14th International Conference on Solid State Sensors, Actuators and Microsystems and the 21st European Conference on Solid-State Transducers
Uncontrolled Keywords:Microfluidics; Magnetic bead separator; SU-8; High-throughput; Soft magnetic elements
Subjects:Analytical Science > Nanotechnology for sensing and actuating
Physical Science > Nanomagnetics
ID Code:4942
Deposited By:SPI
Deposited On:09 Apr 2009 12:51
Last Modified:09 Apr 2009 12:51

Repository Staff Only: item control page