Nano Archive

Ultrasensitive DNA hybridization biosensor based on polyaniline

Arora, Kavita and Prabhakar, Nirmal and Chand, Subhash and Malhotra, B. D. (2007) Ultrasensitive DNA hybridization biosensor based on polyaniline. BIOSENSORS & BIOELECTRONICS, 23 (5). pp. 613-620.

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Abstract

Ultrasensitive DNA hybridization biosensor based on polyaniline (PANI) electrochemically deposited onto Pt disc electrode has been fabricated using biotin-avidin as indirect coupling agent to immobilize single-stranded 5'-biotin end-labeled polydeoxycytidine (BdC) probes and 5'-biotin end-labeled 35 base-long oligonucleotide probe (BdE) to detect complementary target, using both direct electrochemical oxidation of guanine and redox electroactive indicator methylene blue (MB), respectively. These polyaniline-based disc electrodes have been characterized using differential pulse voltammetry (DPV), Fourier transform infrared spectroscopy (FT-IR), impedance measurements and scanning electron microscopy (SEM) techniques, respectively. Compared to direct electrochemical oxidation of guanine, hybridization detection using MB results in the enhanced detection limit by about 100 times. These DNA immobilized PANI electrodes have hybridization response time of about 60 s. (c) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:polyaniline; biotin-avidin; methylene blue; DNA hybridization; biosensor
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanobiotechnology
Material Science > Nanochemistry
ID Code:481
Deposited By:INVALID USER
Deposited On:05 Dec 2008 16:35
Last Modified:14 Aug 2009 12:26

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