Nano Archive

A single cell electrophysiological analysis device with embedded electrode

Li, Sha and Lin, Liwei (2007) A single cell electrophysiological analysis device with embedded electrode. Sensors and Actuators A: Physical, 134 (1). 20 - 26.

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Official URL: http://www.sciencedirect.com/science/article/B6THG...

Abstract

A single cell electrophysiological analysis device with a sub-micrometer opening on embedded recording electrode has been demonstrated using a combined micro- and nano-machining process. This technique can reduce the series pipette resistance while keeping high seal resistance for electrophysiological measurements. In the prototype demonstration, the electrolyte pipette resistance is reduced to 200 kΩ using the microfabricated electrophysiological system with opening size of 500 nm in diameter. A 6 GΩ seal resistance is achieved on a HeLa cell, a cervical cancer cell line with size between 10 and 20 μm, on the same device. In a second experiment, the measured electrolyte pipette resistance is 1 MΩ using different pipette solution on a device with 800 nm opening and a 500 MΩ seal resistance is achieved on a vacuolar yeast cell of 3–5 μm in size.

Item Type:Article
Additional Information:International Mechanical Engineering congress and Exposition 2005 - IMECE 2005, American Society of Mechanical Engineering International Mechanical Engineering Congress and Exposition
Uncontrolled Keywords:Electrophysiological analysis; Patch clamp; Electrolyte pipette resistance; Seal resistance
Subjects:Analytical Science > Nanotechnology for sensing and actuating
ID Code:4786
Deposited By:SPI
Deposited On:07 May 2009 10:48
Last Modified:07 May 2009 10:48

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