Li, Sha and Lin, Liwei (2007) A single cell electrophysiological analysis device with embedded electrode. Sensors and Actuators A: Physical, 134 (1). 20 - 26.
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Official URL: http://www.sciencedirect.com/science/article/B6THG...
A single cell electrophysiological analysis device with a sub-micrometer opening on embedded recording electrode has been demonstrated using a combined micro- and nano-machining process. This technique can reduce the series pipette resistance while keeping high seal resistance for electrophysiological measurements. In the prototype demonstration, the electrolyte pipette resistance is reduced to 200 kΩ using the microfabricated electrophysiological system with opening size of 500 nm in diameter. A 6 GΩ seal resistance is achieved on a HeLa cell, a cervical cancer cell line with size between 10 and 20 μm, on the same device. In a second experiment, the measured electrolyte pipette resistance is 1 MΩ using different pipette solution on a device with 800 nm opening and a 500 MΩ seal resistance is achieved on a vacuolar yeast cell of 3–5 μm in size.
|Additional Information:||International Mechanical Engineering congress and Exposition 2005 - IMECE 2005, American Society of Mechanical Engineering International Mechanical Engineering Congress and Exposition|
|Uncontrolled Keywords:||Electrophysiological analysis; Patch clamp; Electrolyte pipette resistance; Seal resistance|
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
|Deposited On:||07 May 2009 10:48|
|Last Modified:||07 May 2009 10:48|
Repository Staff Only: item control page