Noh, Jermim and Sung, Su Whan and Jeon, Min Ku and Kim, Soo Ho and Lee, Luke P. and Woo, Seong Ihl (2005) In situ thermal diagnostics of the micro-PCR system using liquid crystals. Sensors and Actuators A: Physical, 122 (2). 196 - 202.
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Official URL: http://www.sciencedirect.com/science/article/B6THG...
A simple, reliable liquid crystal (LC) thermometry of a polymerase chain reaction (PCR) chip that has a microchamber inside the chip and a film heater and a film sensor patterned on the surface is firstly introduced. The silicon-based PCR chip is made by MEMS technology. A non-linear feedback proportional-integral control scheme adjusts the power input of the Pt heater to control the surface temperature of the micro-PCR chip measured by the Pt sensor with an accuracy of less than ±0.1 °C at steady state. We use the LC thermometry to analyze the temperature distribution in the microchamber of the micro-PCR chip. The reflected colors of LC's inside the microchamber are captured through a charge-coupled device (CCD) camera, subsequently analyzed using a hue-based color analysis system. Two LC's of which individual working range is near the typical PCR temperature of 55 °C and 72 °C are calibrated in a constant temperature bath with a resolution of less than ±0.3 °C. While the average microchamber temperature near the annealing temperature is similar to the surface temperature, it is lower than the surface temperature by ca. 1 °C in the range of the extension temperature. Also, the temperature variations along the center of the microchamber, are less than 1.5 ± 0.5 °C and 3 ± 0.3 °C in the range of the annealing and the extension temperature, respectively.
|Uncontrolled Keywords:||Liquid crystals; PCR chip; Microfabrication; Temperature uniformity; Hue|
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
|Deposited On:||06 Jul 2009 15:01|
|Last Modified:||06 Jul 2009 15:01|
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