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Development of an SH-SAW sensor for the detection of DNA hybridization

Hur, Youngjune and Han, Jinho and Seon, Jooheon and Pak, Yukeun Eugene and Roh, Yongrae (2005) Development of an SH-SAW sensor for the detection of DNA hybridization. Sensors and Actuators A: Physical, 120 (2). 462 - 467.

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Official URL: http://www.sciencedirect.com/science/article/B6THG...

Abstract

We have developed SH (shear horizontal) surface acoustic wave (SAW) sensors for the detection of DNA (deoxyribonucleic acid) hybridization on the gold-coated delay line of transverse SAW devices. DNA hybridization experiments were performed with 15-mer oligonucleotides (probe and complementary target DNA). The sensor consists of twin SAW delay line oscillators (sensing channel and reference channel) operating at 100 MHz fabricated on 36° rotated Y-cut X-propagation LiTaO3 piezoelectric single crystals. The relative change in the frequency of the two oscillators was monitored to detect hybridization between the target DNA and probe DNA immobilized on the delay line of the SAW sensor. The measurement results showed a good response of the sensor to the mass loading effects of the DNA hybridization with a sensitivity level up to 1.55 ng/ml/Hz.

Item Type:Article
Uncontrolled Keywords:SH-SAW; Oscillator; Oligonucleotide; DNA immobilization; DNA hybridization
Subjects:Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanoscale biological processes
ID Code:4637
Deposited By:SPI
Deposited On:06 Jul 2009 15:20
Last Modified:06 Jul 2009 15:20

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