Nano Archive

Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering

Huang, Yu-Fen and Lin, Yang-Wei and Lin, Zong-Hong and Chang, Huan-Tsung (2009) Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering. Journal of Nanoparticle Research, 11 (4). pp. 775-783.

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Official URL: http://www.springerlink.com/content/g906305160jw15...

Abstract

In this study, we demonstrated the potential use of nucleic acid ligand (aptamers) conjugated gold nanoparticles (AuNPs) for cancer cell detection. Through specific binding of the aptamers toward platelet-derived growth factor (PDGF), MDA-MB-231 and Hs578T cells (cancer cells) that over-express PDGF, interact with Apt-AuNPs to a greater extent than do H184B5F5/M10 cells (normal cells). These results were confirmed through inductively coupled plasma mass spectrometry measurements of the gold ion concentrations within these cells. Aggregation of the Apt-AuNPs in the cytoplasm of the cancer cells led to the generation of an intense scattered light upon photo-illumination; this phenomenon allows the differentiation of cancer cells from normal cells using a dark field optical microscope. The presence of Apt-AuNPs suppressed the proliferation of MDA-MB-231 cancer cells, but not H184B5F5/M10 cells.

Item Type:Article
ID Code:4537
Deposited By:Prof. Alexey Ivanov
Deposited On:08 May 2009 06:21
Last Modified:08 May 2009 09:53

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