Huang, Yu-Fen and Lin, Yang-Wei and Lin, Zong-Hong and Chang, Huan-Tsung (2009) Aptamer-modified gold nanoparticles for targeting breast cancer cells through light scattering. Journal of Nanoparticle Research, 11 (4). pp. 775-783.
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Official URL: http://www.springerlink.com/content/g906305160jw15...
In this study, we demonstrated the potential use of nucleic acid ligand (aptamers) conjugated gold nanoparticles (AuNPs) for cancer cell detection. Through specific binding of the aptamers toward platelet-derived growth factor (PDGF), MDA-MB-231 and Hs578T cells (cancer cells) that over-express PDGF, interact with Apt-AuNPs to a greater extent than do H184B5F5/M10 cells (normal cells). These results were confirmed through inductively coupled plasma mass spectrometry measurements of the gold ion concentrations within these cells. Aggregation of the Apt-AuNPs in the cytoplasm of the cancer cells led to the generation of an intense scattered light upon photo-illumination; this phenomenon allows the differentiation of cancer cells from normal cells using a dark field optical microscope. The presence of Apt-AuNPs suppressed the proliferation of MDA-MB-231 cancer cells, but not H184B5F5/M10 cells.
|Deposited By:||Prof. Alexey Ivanov|
|Deposited On:||08 May 2009 06:21|
|Last Modified:||08 May 2009 09:53|
Repository Staff Only: item control page