Nano Archive

Growth of Gold Nanoparticles in Human Cells

Anshup, J and Venkataraman, J. Sai and Subramaniam, Chandramouli and Kumar, R. Rajeev and Priya, Suma and Kumar,, T. R. Santhosh and Omkumar, R. V. and John, Annie and Pradeep, T. (2005) Growth of Gold Nanoparticles in Human Cells. Langmuir, 21 (25). 11562- 11567.

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Official URL: http://pubs.acs.org/doi/abs/10.1021/la0519249

Abstract

Gold nanoparticles of 20−100 nm diameter were synthesized within HEK-293 (human embryonic kidney), HeLa (human cervical cancer), SiHa (human cervical cancer), and SKNSH (human neuroblastoma) cells. Incubation of 1 mM tetrachloroaurate solution, prepared in phosphate buffered saline (PBS), pH 7.4, with human cells grown to 80% confluency yielded systematic growth of nanoparticles over a period of 96 h. The cells, stained due to nanoparticle growth, were adherent to the bottom of the wells of the tissue culture plates, with their morphology preserved, indicating that the cell membrane was intact. Transmission electron microscopy of ultrathin sections showed the presence of nanoparticles within the cytoplasm and in the nucleus, the latter being much smaller in dimension. Scanning near field microscopic images confirmed the growth of large particles within the cytoplasm. Normal cells gave UV−visible signatures of higher intensity than the cancer cells. Differences in the cellular metabolism of cancer and noncancer cells were manifested, presumably in their ability to carry out the reduction process.

Item Type:Article
Subjects:Material Science > Nanochemistry
Divisions:Faculty of Engineering, Science and Mathematics > School of Chemistry
ID Code:4482
Deposited By:JNCASR
Deposited On:02 Apr 2009 10:43
Last Modified:02 Apr 2009 10:43

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