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The antibacterial properties of a novel chitosan–Ag-nanoparticle composite

Sanpui, Pallab and Murugadoss, A. and Prasad, P. V. Durga and Ghosh, Siddhartha Sankar and Chattopadhyay, Arun (2008) The antibacterial properties of a novel chitosan–Ag-nanoparticle composite. International Journal of Food Microbiology, 124 (2). pp. 142-146.

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Abstract

Escherichia coli expressing recombinant green fluorescent protein was used to test the bactericidal efficacy of a newly synthesized chitosan–Ag-nanoparticle composite. The composite was found to have significantly higher antimicrobial activity than its components at their respective concentrations. The one-pot synthesis method led to the formation of small Ag nanoparticles attached to the polymer which can be dispersed in media of pH ≤ 6.3. The presence of a small percentage (2.15%, w/w) of metal nanoparticles in the composite was enough to significantly enhance inactivation of E. coli as compared with unaltered chitosan. Fluorescence spectroscopy indicated that bacterial growth stopped immediately after exposure of E. coli to the composite, with release of cellular green fluorescent protein into the medium at a faster rate than with chitosan. Fluorescence confocal laser scanning and scanning electron microscopy showed attachment of the bacteria to the composite and their subsequent fragmentation. Native protein gel electrophoresis experiments indicated no effect of the composite on bacterial proteins.

Item Type:Article
Subjects:Material Science > Bio materials
Material Science > Nanochemistry
Divisions:Faculty of Engineering, Science and Mathematics > School of Chemistry
ID Code:4355
Deposited By:JNCASR
Deposited On:03 Apr 2009 05:22
Last Modified:09 Apr 2009 05:37

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