Panda, Biswa Ranjan and Singh, Atul Kumar and Ramesh, Aiyagari and Chattopadhyay, Arun (2008) Rapid Estimation of Bacteria by a Fluorescent Gold Nanoparticle−Polythiophene Composite. Langmuir, 24 (20). pp. 11995-12000.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/la802171b
Abstract
Herein we present a facile method for rapid quantitation of bacterial cells over several logarithmic dilutions. The quantitation is based on loss of the fluorescence intensity of a positively charged Au nanoparticle−polythiophene composite in the presence of bacterial cells. The present method allowed estimation of both Gram-positive and Gram-negative bacteria with cells as low as 1000. Transmission electron microscopic investigations revealed attachment of the composite with bacteria with no discernible change in the morphology of the cells. Further, dynamic light scattering experiments indicated preferential attachment of smaller composite particles over larger ones, which were also attached at higher bacterial concentrations. The ease of operation with minimal sample manipulation steps, high sensitivity, quantitative detection, and its generality offer specific advantages over conventional methods.
| Item Type: | Article |
|---|---|
| Subjects: | Material Science > Nanochemistry |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Chemistry |
| ID Code: | 4348 |
| Deposited By: | JNCASR |
| Deposited On: | 03 Apr 2009 05:22 |
| Last Modified: | 03 Apr 2009 05:22 |
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