Chen, Xiao and Zhang, Jing Jing and Xuan, Jie and Zhu, Jun Jie (2009) Myoglobin/gold nanoparticles/carbon spheres 3-D architecture for the fabrication of a novel biosensor. Nano Research, 2 (3). pp. 210-219.
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Official URL: http://www.springerlink.com/content/l2575817677j11...
A novel biosensor based on a myoglobin/gold nanoparticles/carbon spheres (Mb-AuNPs-CNs) 3-D architecture bioconjunction has been fabricated for the determination of hydrogen peroxide (H2O2). Cyclic voltammetry (CV), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) were used to characterize the bioconjunction of the AuNPs-CNs with Mb. Experimental results demonstrate that the AuNPs-CNs hybrid material is more effective in facilitating electron transfer of the immobilized enzyme than CNs alone, which can be attributed to the unique nanostructure and larger surface area of the bioconjunction. The biosensor displayed good performance for the detection of H2O2 with a wide linear range from 0.28 μmol/L to 116.5 μmol/L and a detection limit of 0.12 μmol/L. The Michaelis-Menten constant K Mapp value was estimated to be 0.3 mmol/L. The resulting biosensor exhibited fast amperometric response, and good stability, reproducibility, and selectivity to H2O2.
|Deposited By:||Prof. Alexey Ivanov|
|Deposited On:||16 Mar 2009 09:42|
|Last Modified:||11 Aug 2009 15:51|
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