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Effects of Proteins from Culture Medium on Surface Property of Silanes- Functionalized Magnetic Nanoparticles

Chen, Z. P. and Xu, R. Z. and Zhang, Y. and Gu, N. (2009) Effects of Proteins from Culture Medium on Surface Property of Silanes- Functionalized Magnetic Nanoparticles. Nanoscale Research Letters, 4 (3). pp. 204-209.

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Monodisperse magnetic nanoparticles (MNPs) were synthesized by thermal decomposition of iron-oleate and functionalized with silanes bearing various functional groups such as amino group (NH2), short-chain poly(ethylene glycol) (PEG), and carboxylic group (COOH). Then, silanes-functionalized magnetic nanoparticles (silanes-MNPs) were incubated in cell culture medium plus fetal calf serum to investigate the effects of proteins from culture medium on surface property of MNPs. Zeta potential measurements showed that although surface charges of silanes-MNPs were different, they exhibited negative charges at neutral pH and approximate isoelectric points after they were incubated in cell culture medium. The reason was that silanes-MNPs could easily adsorb proteins from culture medium via non-covalent binding, resulting in the formation of protein-silanes-MNPs conjugates. Moreover, silanes-MNPs with various functional groups had different adsorption capacity to proteins, as confirmed by Coomassie blue fast staining method. The in vitro cell experiments showed that protein-silanes-MNPs had higher cellular uptake by cancer cells than silanes-MNPs.

Item Type:Article
Uncontrolled Keywords:Magnetic nanoparticles - Culture medium - Protein - Surface property - Cellular uptake
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanobiotechnology
Physical Science > Nanomagnetics
Biomedical Science > Nanomedicine
ID Code:4186
Deposited By:Lesley Tobin
Deposited On:05 Aug 2009 15:56
Last Modified:05 Aug 2009 15:56

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