Nano Archive

Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging

Ge, Yuqing and Zhang, Yu and He, Shiying and Nie, Fang and Teng, Gaojun and Gu, Ning (2009) Fluorescence Modified Chitosan-Coated Magnetic Nanoparticles for High-Efficient Cellular Imaging. Nanoscale Research Letters, 4 (4). pp. 287-295.

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Labeling of cells with nanoparticles for living detection is of interest to various biomedical applications. In this study, novel fluorescent/magnetic nanoparticles were prepared and used in high-efficient cellular imaging. The nanoparticles coated with the modified chitosan possessed a magnetic oxide core and a covalently attached fluorescent dye. We evaluated the feasibility and efficiency in labeling cancer cells (SMMC-7721) with the nanoparticles. The nanoparticles exhibited a high affinity to cells, which was demonstrated by flow cytometry and magnetic resonance imaging. The results showed that cell-labeling efficiency of the nanoparticles was dependent on the incubation time and nanoparticles'€™ concentration. The minimum detected number of labeled cells was around 104 by using a clinical 1.5-T MRI imager. Fluorescence and transmission electron microscopy instruments were used to monitor the localization patterns of the magnetic nanoparticles in cells. These new magneto-fluorescent nanoagents have demonstrated the potential for future medical use.

Item Type:Article
Uncontrolled Keywords:Magnetic nanoparticle - Fluorescence - Chitosan - Magnetic resonance imaging
Subjects:Biomedical Science > Nanobiotechnology
Physical Science > Nanomagnetics
Biomedical Science > Nanomedicine
ID Code:4154
Deposited By:Lesley Tobin
Deposited On:14 Aug 2009 14:43
Last Modified:14 Aug 2009 14:43

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