Tripathi, VS and Kandimalla, VB and Ju, HX (2006) Amperometric biosensor for hydrogen peroxide based on ferrocene-bovine serum albumin and multiwall carbon nanotube modified ormosil composite. BIOSENSORS & BIOELECTRONICS, 21 (8). pp. 1529-1535.
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A novel amperometric biosensor for hydrogen peroxide (H2O2) was developed by entrapping horseradish peroxidase (HRP) in a new ormosil composite doped with ferrocene monocarboxylic acid-bovine serum albumin conjugate and multiwall carbon nanotubes (MWNTs). The ormosil was prepared using 3-(aminopropyl)triethoxysilane and 2-(3,4 epoxycyclohexyl)-ethyltrimethoxy silane as monomers. The encapsulated conjugate showed excellent electrochemistry and acted as an electron transfer mediator. The presence of MWNTs improved the conductivity of the composite film. This matrix showed a biocompatible microenvironment for retaining the native activity of the entrapped HRP and a very low mass transport barrier to the substrate, which provided a fast amperometric response to H2O2. The proposed H2O2 biosensor exhibited a linear ran-e of 0.02-4.0 mM with a detection limit of 5.0 mu M (S/N = 3) and a K-M(app) value of 2.0 mM. It could be used for flow injection analysis of hydrogen peroxide with a liner range from 0.02 to 4.5 mM, sensitivity of 0.042 mu A/mM and analytical time of 20 s per sample. This biosensor possessed good analytical performance and storage stability. (c) 2005 Elsevier B.V. All rights reserved.
|Uncontrolled Keywords:||ormosil; ferrocene monocarboxylic acid-bovine serum albumin conjugate; multiwall carbon nanotubes; horseradish peroxidase; hydrogen peroxide; biosensors|
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
Biomedical Science > Nanobiotechnology
|Deposited On:||23 Jan 2009 09:40|
|Last Modified:||23 Jan 2009 09:40|
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