Nano Archive

Electrochemical detection of DNA hybridization amplified by nanoparticles

Peng, H and Soeller, C and Cannell, M. B. and Bowmaker, G. A. and Cooney, R. P. and Travas-Sejdic, J (2006) Electrochemical detection of DNA hybridization amplified by nanoparticles. BIOSENSORS & BIOELECTRONICS, 21 (9). pp. 1727-1736.

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Detection of specific oligonucleotide (ODN) fragments has become an important field in many areas of biomedicine. We describe a novel ODN sensor based on electropolymerization of a conducting polymer (polypyrrole) in the presence of a sample containing ODN(s). The resulting trapped ODN(s) are then probed by addition of complimentary sequence ODN. By incorporating Us nanoparticles with the probe, a significant improvement in sensor sensitivity was observed. Impedance spectroscopy suggested that optimal detection of hybridization occurred at frequencies >= 3000 Hz (for a 0.07 cm(2) 85 nin thick film). At these frequencies, the impedance signal was almost linear with the logarithm of ODN concentration in the range 3.7-370 nM with a detection limit of similar to 1 nM ODN (for the sensor fabricated). Importantly, the sensor could be regenerated by removing hybridized ODN with NaOH suggesting possibility of the sensor re-use. (c) 2005 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:DNA sensor; nanoparticles; polypyrrole; electrochemical impedance spectroscopy
Subjects:Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanobiotechnology
Material Science > Nanostructured materials
ID Code:3701
Deposited By:Farnush Anwar
Deposited On:22 Jan 2009 12:12
Last Modified:13 Feb 2009 09:07

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