Lee, Jae-Seung and Ulmann, Pirmin A. and Han, Min Su and Mirkin, Chad A. (2008) A DNA-gold nanoparticle-based colorimetric competition assay for the detection of cysteine. NANO LETTERS, 8 (2). pp. 529-533.
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Official URL: http://pubs.acs.org/doi/abs/10.1021/nl0727563
Abstract
We report the development of a highly sensitive and selective colorimetric detection method for cysteine based upon oligonucleotide-functionalized gold nanoparticle probes that contain strategically placed thymidine-thymidine (T-T) mismatches complexed with Hg2+. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the sharp melting transition of oligonucleotide-linked nanoparticle aggregates, and the very selective coordination of Hg2+ with cysteine. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer the temperature at which the purple-to-red color change associated with aggregate dissociation takes place. This assay does not utilize organic cosolvents, enzymatic reactions, light-sensitive dye molecules, lengthy protocols, or sophisticated instrumentation thereby overcoming some of the limitations of more conventional methods.
| Item Type: | Article |
|---|---|
| Subjects: | Biomedical Science > Nanobiotechnology Biomedical Science > Nanomedicine |
| ID Code: | 3536 |
| Deposited By: | Farnush Anwar |
| Deposited On: | 16 Jan 2009 16:47 |
| Last Modified: | 29 Jan 2009 11:41 |
Repository Staff Only: item control page
