Doria, G and Franco, R and Baptista, P (2007) Nanodiagnostics: fast colorimetric method for single nucleotide polymorphism/mutation detection. IET NANOBIOTECHNOLOGY, 1 (4). pp. 53-57.
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Official URL: http://dx.doi.org/10.1049/iet-nbt:20070001
Advances in nanosciences are having a significant impact in many areas of research. The impact of new nanotechnologies has been particularly large in biodiagnostics, where a number of nanoparticle-based assays have been introduced for biomolecules detection. To date, applications of nanoparticles have largely focused on DNA-functionalised gold nanoparticles used as the target-specific probes. These gold nanoparticle-based systems can be used for the detection of specific sequences of DNA (pathogen detection, characterisation of mutation and/or single nucleotide polymorphisms) or RNA (without prior retro-transcription and amplification). Here a rapid and inexpensive nanoparticle-based method for single-base mismatch detection (single nucleotide polymorphism/mutation) in DNA samples is reported. Gold nanoparticles derivatised with thiol modified oligonucleotides complementary to DNA targets - Au-nanoprobes - are used to distinguish fully complementary from mismatched sequences, with a single-base mismatch. The authors have successfully applied this strategy to detect common mutations within the beta-globin gene.
|Subjects:||Biomedical Science > Nanobiotechnology|
Biomedical Science > Nanomedicine
|Deposited By:||Farnush Anwar|
|Deposited On:||15 Jan 2009 12:52|
|Last Modified:||12 Feb 2009 14:28|
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