Polsky, Ronen and Harper, Jason C. and Wheeler, David R. and Dirk, Shawn M. and Arango, Dulce C. and Brozik, Susan M. (2008) Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications. BIOSENSORS & BIOELECTRONICS, 23 (6). pp. 757-764.
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Official URL: http://dx.doi.org/10.1016/j.bios.2007.08.013
The direct electrically addressable deposition of diazonium-modified antibodies is examined for electrochemical immunosensing applications. The immobilized antibodies can be detected by the use of electroactive enzyme tags and nanoparticle-gold labeling. Control over antibody functionalization density and minimal spontaneous grafting of diazonium-antibody adducts is shown. The utility of the technique for a sandwich immunoassay as well as the ability to individually and selectively address closely spaced microelectrodes for multi-target protein detection in an array format is demonstrated. (C) 2007 Elsevier B.V. All rights reserved.
|Uncontrolled Keywords:||immunosensors; electrochemical biosensor; diazonium; electrode array; selective functionalization; multianalyte detection|
|Subjects:||Biomedical Science > Nanobiotechnology|
Biomedical Science > Nanomedicine
|Deposited By:||Farnush Anwar|
|Deposited On:||15 Jan 2009 10:52|
|Last Modified:||27 Jan 2009 14:30|
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