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Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications

Polsky, Ronen and Harper, Jason C. and Wheeler, David R. and Dirk, Shawn M. and Arango, Dulce C. and Brozik, Susan M. (2008) Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications. BIOSENSORS & BIOELECTRONICS, 23 (6). pp. 757-764.

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Official URL: http://dx.doi.org/10.1016/j.bios.2007.08.013

Abstract

The direct electrically addressable deposition of diazonium-modified antibodies is examined for electrochemical immunosensing applications. The immobilized antibodies can be detected by the use of electroactive enzyme tags and nanoparticle-gold labeling. Control over antibody functionalization density and minimal spontaneous grafting of diazonium-antibody adducts is shown. The utility of the technique for a sandwich immunoassay as well as the ability to individually and selectively address closely spaced microelectrodes for multi-target protein detection in an array format is demonstrated. (C) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:immunosensors; electrochemical biosensor; diazonium; electrode array; selective functionalization; multianalyte detection
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:3256
Deposited By:Farnush Anwar
Deposited On:15 Jan 2009 10:52
Last Modified:27 Jan 2009 14:30

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