Slowing, Igor I. and Trewyn, Brian G. and Lin, Victor S. -Y. (2007) Mesoporous silica nanoparticles for intracellular delivery of membrane-impermeable proteins. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 129 (28). pp. 8845-8849.
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Official URL: http://dx.doi.org/10.1021/ja0719780
An MCM-41-type mesoporous silica nanoparticle (MSN) material with a large average pore diameter (5.4 nm) is synthesized and characterized. The in vitro uptake and release profiles of cytochrome c by the MSN were investigated. The enzymatic activity of the released protein was quantitatively analyzed and compared with that of the native cytochrome c in physiological buffer solutions. We found that the enzymes released from the MSNs are still functional and highly active in catalyzing the oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) by hydrogen peroxide. In contrast to the fact that cytochrome c is a cell-membrane-impermeable protein, we discovered that the cytochrome c-encapsulated MSNs could be internalized by live human cervical cancer cells (HeLa) and the protein could be released into the cytoplasm. We envision that these MSNs with large pores could serve as a transmembrane delivery vehicle for controlled release of membrane-impermeable proteins in live cells, which may lead to many important biotechnological applications including therapeutics and metabolic manipulation of cells.
|Subjects:||Physical Science > Nano objects|
Biomedical Science > Nanobiotechnology
Material Science > Nanochemistry
Material Science > Nanostructured materials
|Deposited By:||Anuj Seth|
|Deposited On:||13 Jan 2009 11:14|
|Last Modified:||20 Jan 2009 14:42|
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