Nano Archive

In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells

Davoren, Maria and Herzog, Eva and Casey, Alan and Cottineau, Benjamin and Chambers, Gordon and Byrne, Hugh J. and Lyng, Fiona M. (2007) In vitro toxicity evaluation of single walled carbon nanotubes on human A549 lung cells. TOXICOLOGY IN VITRO, 21 (3). pp. 438-448.

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This paper describes the in vitro cytotoxicity assessment of single walled carbon nanotubes (SWCNT) on A549 cells, a human lung cell line. Cellular viability was determined using the alamar blue (AB), neutral red (NR) and MTT assays, which evaluated metabolic, lysosomal and mitochondrial activity respectively. In addition, the total protein content of the cells was measured using the coomassie brilliant (CB) blue assay. Supernatants were also assayed for Adenylate Kinase (AK) release and Interleukin 8 (IL-8) which indicated a loss of cell membrane integrity and an inflammation response respectively. To investigate the interactions between serum components in the test medium and the test materials, exposures were conducted both in serum containing (5%) and serum-free medium. Results from the cytotoxicity tests (AB, CB, MTT) revealed the SWCNT to have very low acute toxicity to the A549 cells as all but one of the reported 24 h EC50 values exceeded the top concentration tested (800 mu g/ml). The SWCNT were found to interfere with a number of the dyes used in the cytotoxicity assessment and we are currently conducting a comprehensive spectroscopic study to further investigate these interactions. Of the multiple cytotoxicity assays used, the AB assay was found to be the most sensitive and reproducible. Transmission electron microscopy (TEM) studies confirmed that there was no intracellular localization of SWCNT in A549 cells following 24 h exposure; however, increased numbers of surfactant storing lamellar bodies were observed in exposed cells. (c) 2006 Elsevier Ltd. All rights reserved.

Item Type:Article
Uncontrolled Keywords:single walled carbon nanotubes; cytotoxicity; TEM; foetal bovine serum; lamellar bodies
Subjects:Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
Biomedical Science > Nanotechnology for animal health
NanoSafety > Environment, health and safety aspects of nanotechnology
ID Code:3081
Deposited By:Anuj Seth
Deposited On:13 Jan 2009 12:25
Last Modified:20 Jan 2009 14:50

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