Kolsenik, Jenny and Belgorodsky, Bogdan and Fadeev, Ludmila and Gozin, Michael (2007) Can apomyoglobin form a complex with a spherical ligand? Interactions between apomyoglobin and [C-60] fullerene derivative. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 7 (4-5). pp. 1389-1394.
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The preparation and characterization of the stable equine skeletal muscle apomyoglobin and eee-isomer of tris-malonic acid [C-60] fullerene complex is reported. For this new bio-nanomaterial preparation, a procedure of complexation-during-protein-refolding was used and the obtained compound sustained separation by gel-filtration and ion-exchange chromatography. The apomyoglobin-tris-malonic acid [C-60] fullerene complex was characterized by UV-vis spectroscopy, steady state fluorescence, time-resolved fluorescence, and circular dichroism spectroscopies. Important information provided by this study, regarding the stability and properties of new material, may lead to a better understanding of the apomyoglobin protein binding characteristics, as well as to development of novel antioxidant and photodynamic therapeutic agents and components for bioelectronic devices.
|Uncontrolled Keywords:||apomyoglobin; [C-60]fullerene; complex|
|Subjects:||Biomedical Science > Nanobiotechnology|
Material Science > Bio materials
|Deposited By:||Farnush Anwar|
|Deposited On:||13 Jan 2009 12:22|
|Last Modified:||26 Jan 2009 16:07|
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