Nano Archive

Penetration of fullerene C60 derivatives through biological membranes

Andreev, Igor and Petrukhina, Anna and Garmanova, Alla and Babakhin, Alexander and Andreev, Sergey and Romanova, Valentina and Troshin, Pavel and Troshina, Olesya and DuBuske, Lawrence (2008) Penetration of fullerene C60 derivatives through biological membranes. FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES, 16 (2). pp. 89-102.

Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.

Official URL: http://www.informaworld.com/smpp/content~content=a...

Abstract

Penetration of fullerene C-60 in hydrated molecular-colloidal form (FMC) and various C-60 water-soluble derivatives (FDs) through membranes of human erythrocytes, platelets and symbiosomes (subcellular organelles of plant origin) were tested. The FDs bearing amino acids induced pronounced depolarization of symbiosome membranes energized with Mg-ATP. In erythrocytes and platelets incubated in K+-free medium in the presence of FCCP, FDs with malonic acid pendants promoted acidification of the intracellular medium thereby simulating an effect of the K+ ionophore valinomycin. Dissipation of Delta pH artificially induced on the plasma membrane of these cells was observed in the presence of C-60-gamma-aminobutiric acid which, in addition, strongly stimulated Mg-ATP-dependent generation of membrane potential on symbiosome membranes. C-60-Arg was shown to dissipate K+-diffusion potential on erythrocyte membranes induced by valinomycin. Fullerene C-60 used in hydrated molecular-colloidal form (FMC) also entered symbiosomes and platelets as evidenced by the quenching of the fluorescence of the Ca2+ indicator chlorotetracycline localized in the interior of these cells. These findings provide evidence for ease of permeation of these fullerene-based compounds through biological membranes from different type cells.

Item Type:Article
Uncontrolled Keywords:fullerene C-60; C-60 amino acid derivatives; fluorescent probe; membrane potential
Subjects:Material Science > Functional and hybrid materials
Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
ID Code:2882
Deposited By:Farnush Anwar
Deposited On:12 Jan 2009 12:29
Last Modified:23 Jan 2009 11:11

Repository Staff Only: item control page