Andreev, Igor and Petrukhina, Anna and Garmanova, Alla and Babakhin, Alexander and Andreev, Sergey and Romanova, Valentina and Troshin, Pavel and Troshina, Olesya and DuBuske, Lawrence (2008) Penetration of fullerene C60 derivatives through biological membranes. FULLERENES NANOTUBES AND CARBON NANOSTRUCTURES, 16 (2). pp. 89-102.
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Penetration of fullerene C-60 in hydrated molecular-colloidal form (FMC) and various C-60 water-soluble derivatives (FDs) through membranes of human erythrocytes, platelets and symbiosomes (subcellular organelles of plant origin) were tested. The FDs bearing amino acids induced pronounced depolarization of symbiosome membranes energized with Mg-ATP. In erythrocytes and platelets incubated in K+-free medium in the presence of FCCP, FDs with malonic acid pendants promoted acidification of the intracellular medium thereby simulating an effect of the K+ ionophore valinomycin. Dissipation of Delta pH artificially induced on the plasma membrane of these cells was observed in the presence of C-60-gamma-aminobutiric acid which, in addition, strongly stimulated Mg-ATP-dependent generation of membrane potential on symbiosome membranes. C-60-Arg was shown to dissipate K+-diffusion potential on erythrocyte membranes induced by valinomycin. Fullerene C-60 used in hydrated molecular-colloidal form (FMC) also entered symbiosomes and platelets as evidenced by the quenching of the fluorescence of the Ca2+ indicator chlorotetracycline localized in the interior of these cells. These findings provide evidence for ease of permeation of these fullerene-based compounds through biological membranes from different type cells.
|Uncontrolled Keywords:||fullerene C-60; C-60 amino acid derivatives; fluorescent probe; membrane potential|
|Subjects:||Material Science > Functional and hybrid materials|
Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
|Deposited By:||Farnush Anwar|
|Deposited On:||12 Jan 2009 12:29|
|Last Modified:||23 Jan 2009 11:11|
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