Nano Archive

Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro

Ni, Jin and Wu, Qiuye and Li, Yuguo and Guo, Zhixin and Tang, Gusheng and Sun, Ding and Gao, Fu and Cai, Jianming (2008) Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro. JOURNAL OF NANOPARTICLE RESEARCH, 10 (4). pp. 643-651. ISSN 1388-0764 (Print) 1572-896X (Online)

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Official URL: http://www.springerlink.com/content/u5604g78n1ml61...

Abstract

There is growing evidence in recent years that the pristine fullerene may be endowed with strong pro-oxidant capacity to biological samples. In this investigation we tested the hypothesis that water-soluble fullerene-C-60 (nano-C-60) may interact with ionizing radiation enhancing its antiproliferative effects. The two tumor cell lines with different radiosensitivity B16 and SMMU-7721 were treated by a combination of pristine fullerene and Co-60 gamma irradiation. We measured cell survival rates, apoptotic characteristics, reactive oxygen species (ROS) production and alteration of cell diameter with or without gamma-irradiation. There was reduced survival with B16 and SMMU-7721 cells exposed to nano-C-60, with the inhibitory concentrations reducing the viability by 50% to 65 part per billion (ppb) and 150 ppb respectively. For cells exposed to nano-C-60 prior to gamma-irradiation, damage to cell membranes and increased numbers of apoptotic cells were detected by morphologic Hoechst-staining analysis and Annexin V/propidium iodide double-staining. In cells exposed to nano-C-60, there were increased levels of ROS, as measured by fluorescence detection under laser confocal microscopy. Preincubation with non-toxic pristine C-60 before gamma-ray caused enlargement of cells with increased diameter. The results show that nano-C-60 inhibits the growth of tumor cells at certain concentrations and increases the effects of Co-60 gamma-irradiation, possibly through the elevated production of cellular ROS and the membrane disruption. Data in this study indicates a possible consideration of using C-60 as a candidate of sensitization modifier in tumor radiation biology.

Item Type:Article
Uncontrolled Keywords:nano-C-60; Co-60 gamma-irradiation; cytotoxicity; ROS; radiosensitization; nanomedicine; health implications
Subjects:Physical Science > Nano objects
Risk > Environment, health and safety aspects of nanotechnology
Biomedical Science > Nanomedicine
ID Code:2831
Deposited By:Farnush Anwar
Deposited On:13 Jan 2009 12:10
Last Modified:13 Jan 2009 12:10

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