Ni, Jin and Wu, Qiuye and Li, Yuguo and Guo, Zhixin and Tang, Gusheng and Sun, Ding and Gao, Fu and Cai, Jianming (2008) Cytotoxic and radiosensitizing effects of nano-C-60 on tumor cells in vitro. JOURNAL OF NANOPARTICLE RESEARCH, 10 (4). pp. 643-651. ISSN 1388-0764 (Print) 1572-896X (Online)
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Official URL: http://www.springerlink.com/content/u5604g78n1ml61...
There is growing evidence in recent years that the pristine fullerene may be endowed with strong pro-oxidant capacity to biological samples. In this investigation we tested the hypothesis that water-soluble fullerene-C-60 (nano-C-60) may interact with ionizing radiation enhancing its antiproliferative effects. The two tumor cell lines with different radiosensitivity B16 and SMMU-7721 were treated by a combination of pristine fullerene and Co-60 gamma irradiation. We measured cell survival rates, apoptotic characteristics, reactive oxygen species (ROS) production and alteration of cell diameter with or without gamma-irradiation. There was reduced survival with B16 and SMMU-7721 cells exposed to nano-C-60, with the inhibitory concentrations reducing the viability by 50% to 65 part per billion (ppb) and 150 ppb respectively. For cells exposed to nano-C-60 prior to gamma-irradiation, damage to cell membranes and increased numbers of apoptotic cells were detected by morphologic Hoechst-staining analysis and Annexin V/propidium iodide double-staining. In cells exposed to nano-C-60, there were increased levels of ROS, as measured by fluorescence detection under laser confocal microscopy. Preincubation with non-toxic pristine C-60 before gamma-ray caused enlargement of cells with increased diameter. The results show that nano-C-60 inhibits the growth of tumor cells at certain concentrations and increases the effects of Co-60 gamma-irradiation, possibly through the elevated production of cellular ROS and the membrane disruption. Data in this study indicates a possible consideration of using C-60 as a candidate of sensitization modifier in tumor radiation biology.
|Uncontrolled Keywords:||nano-C-60; Co-60 gamma-irradiation; cytotoxicity; ROS; radiosensitization; nanomedicine; health implications|
|Subjects:||Physical Science > Nano objects|
Risk > Environment, health and safety aspects of nanotechnology
Biomedical Science > Nanomedicine
|Deposited By:||Farnush Anwar|
|Deposited On:||13 Jan 2009 12:10|
|Last Modified:||13 Jan 2009 12:10|
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