Liu, Y and Qu, X. H. and Guo, H. W. and Chen, H. J. and Liu, B. F. and Dong, S. J. (2006) Facile preparation of amperometric laccase biosensor with multifunction based on the matrix of carbon nanotubes-chitosan composite. BIOSENSORS & BIOELECTRONICS, 21 (12). pp. 2195-2201.
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Official URL: doi:10.1016/j.bios.2005.11.014
The carbon nanotubes-chitosan (CNTs-CS) composite provides a suitable biosensing matrix due to its good conductivity, high stability, and good biocompatibility. Enzymes can be firmly incorporated into the matrix without the aid of other cross-linking reagents. The composite is easy to form insoluble film in solution above pH 6.3. Based on this, a facilely fabricated amperometric biosensor by entrapping laccase into the CNTs-CS composite film has been developed. At pH 6.0, the fungi laccase incorporated into the composite film remains better catalytic activity than that dissolved in solution. The system is in favor of the accessibility of substrate to the active site of laccase, thus the affinity to substrates is improved greatly, such as 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), catechol, and 0, with K. values of 19.86 mu M, 9.43 mu M, and 3.22 mM, respectively. The major advantages of the as-prepared biosensor are: detecting different substrates (ABTS, catechol, and 02), possessing high affinity and sensitivity, durable long-term stability, and facile preparation procedure. On the other hand, the system can be applied in fabrication of biofuel cells as the cathodic catalysts based on its good electrocatalysis for oxygen reduction. It can be extended to immobilize other enzymes and biomolecules, which will greatly facilitate the development of biosensors, biofuel cells, and other bioelectrochemical devices. (c) 2005 Elsevier B.V. All rights reserved.
|Uncontrolled Keywords:||carbon nanotubes; chitosan; laccase; biosensor; Enzyme ; Oxidoreductases ; Composite material ; Chitosan ; Nanotube ; Carbon ; Biosensor ; Laccase ; Amperometry|
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
Biomedical Science > Nanobiotechnology
|Deposited By:||Lesley Tobin|
|Deposited On:||08 Jan 2009 15:25|
|Last Modified:||19 Feb 2009 09:34|
Repository Staff Only: item control page