Nano Archive

Multicolor FRET silica nanoparticles by single wavelength excitation

Wang, L and Tan, WH (2006) Multicolor FRET silica nanoparticles by single wavelength excitation. NANO LETTERS, 6 (1). pp. 84-88.

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Fluorescent nanoparticles with multiple emission signatures by a single wavelength excitation are needed in multiplex bioanalysis and molecular imaging. We have prepared silica nanoparticles encapsulated with three organic dyes using a modified Stober synthesis method. By varying the doping ratio of the three tandem dyes, fluorescence resonance energy transfer (FRET)-mediated emission signatures can be tuned to have the nanoparticles exhibit multiple colors under one single wavelength excitation. These nanoparticles are intensely fluorescent, highly photostable, uniform in size, and biocompatible. The acceptor emission of the FRET nanoparticles has generated a large Stokes shift, which implicates broad applications in biological labeling and imaging. Molecular recognition moieties, such as biotin, can be covalently attached to the nanoparticle surface to allow for specific binding to target molecules. These multicolor FRET silica nanoparticles can be used as barcoding tags for multiplexed signaling. By using these NPs, one can envision a dynamic, multicolor, colocalization methodology to follow proteins, nucleic acids, molecular machines, and assemblies within living systems.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:2752
Deposited By:Lesley Tobin
Deposited On:19 Jan 2009 15:36
Last Modified:19 Jan 2009 15:36

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