Dumortier, Helene and Lacotte, Stephanie and Pastorin, Giorgia and Marega, Riccardo and Wu, Wei and Bonifazi, Davide and Briand, Jean-Paul and Prato, Maurizio and Muller, Sylviane and Bianco, Alberto (2006) Functionalized carbon nanotubes are non-cytotoxic and preserve the functionality of primary immune cells. NANO LETTERS, 6 (7). pp. 1522-1528.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl061160x
Carbon nanotubes are emerging as innovative tools in nanobiotechnology. However, their toxic effects on environment and health have become an issue of strong concern. In the present study, we address the impact of functionalized carbon nanotubes (f-CNTs) on cells of the immune system. We have prepared two types of f-CNTs, following the 1,3-dipolar cycloaddition reaction ( f-CNTs 1 and 2) and the oxidation/ amidation treatment (f-CNTs 3 and 4), respectively. We have found that both types of f-CNTs are uptaken by B and T lymphocytes as well as macrophages in vitro, without affecting cell viability. Subsequently, the functionality of the different cells was analyzed carefully. We discovered that f-CNT 1, which is highly water soluble, did not influence the functional activity of immunoregulatory cells. f-CNT 3, which instead possesses reduced solubility and forms mainly stable water suspensions, preserved lymphocytes' functionality while provoking secretion of proinflammatory cytokines by macrophages.
|Subjects:||Risk > Environment, health and safety aspects of nanotechnology|
|Deposited By:||Lesley Tobin|
|Deposited On:||20 Jan 2009 11:20|
|Last Modified:||20 Jan 2009 11:20|
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