Nano Archive

Biocompatible, biodegradable and sterically stabilized phospholipid nanomicelles improve cryopreservation of oral keratinocytes: A preliminary investigation

UNSPECIFIED (2007) Biocompatible, biodegradable and sterically stabilized phospholipid nanomicelles improve cryopreservation of oral keratinocytes: A preliminary investigation. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 338 (1-2). pp. 333-335.

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Official URL: doi:10.1016/j.ijpharm.2007.02.002

Abstract

In this proof-of principle study, we determined whether biocompatible, biodegradable and sterically stabilized phospholipid nanomicelles (SSNMs) improve viability and membrane integrity of cryopreserved oral keratinocytes. Cultured chemically transformed hamster oral keratinocytes were frozen gradually with and stored in liquid nitrogen in the presence of 10% dimethylsulfoxide (DMSO) or SSNMs composed of distearoylphosphatidyl-ethanolamine-N-poly(ethylene glycol) 2000 (size, 17 +/- 1 nm; 0.1 and 1.0 nmol). Forty-eight hours later, cells were thawed and their viability was determined. Lactate dehydrogenase (LDH) activity in cell lysates and supernatants was quantified as well. SSNMs evoked a significant, concentration-dependant increase in cell viability in comparison to 10% DMSO (p < 0.05). There was also a significant decrease in LDH activity in the supernatant of cells cryopreserved with SSNMs in comparison to 10% DMSO (p < 0.05). These data indicate that SSNMs improve cryopreservation of oral keratinocyte by promoting cell viability and plasma membrane integrity. We suggest that SSNMs should be further developed as a novel nanocryopreservative for keratinocytes. (c) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:nanomedicine; nanobiotechnology; DSPE-PEG; dimethylsulfoxide; LDH; cell viability
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:2719
Deposited By:Farnush Anwar
Deposited On:09 Jan 2009 10:49
Last Modified:09 Jan 2009 10:49

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