Deng, Yang and Wang, Yini and Holtz, Bryan and Li, Jingyi and Traaseth, Nathan and Veglia, Gianluigi and Stottrup, Benjamin J. and Elde, Robert and Pei, Duanqing and Guo, Athena and Zhu, X. -Y. (2008) Fluidic and air-stable supported lipid bilayer and cell-mimicking microarrays. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 130 (19). pp. 6267-6271.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/ja800049f
As drug delivery, therapy, and medical imaging are becoming increasingly cell-specific, there is a critical need for high fidelity and high-throughput screening methods for cell surface interactions. Cell membrane-mimicking surfaces, i.e., supported lipid bilayers (SLBs), are currently not sufficiently robust to meet this need. Here we describe a method of forming fluidic and air-stable SLBs through tethered and dispersed cholesterol groups incorporated into the bottom leaflet. Achieving air stability allows us to easily fabricate SLB microarrays from direct robotic spotting of vesicle solutions. We demonstrate their application as cell membrane-mimicking microarrays by reconstituting peripheral as well as integral membrane components that can be recognized by their respective targets. These demonstrations establish the viability of the fluidic and air-stable SLB platform for generating content microarrays in high throughput studies, e.g., the screening of drugs and nanomedicine targeting cell surface receptors.
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited By:||Farnush Anwar|
|Deposited On:||09 Jan 2009 11:59|
|Last Modified:||09 Jan 2009 11:59|
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