Lim, Sok Bee and Rubinstein, Israel and Onyuksel, Hayat (2008) Freeze drying of peptide drugs self-associated with long-circulating, biocompatible and biodegradable sterically stabilized phospholipid nanomicelles. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 356 (1-2). pp. 345-350.
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Official URL: http://dx.doi.org/10.1016/j.ijpharm.2008.01.014
The purpose of this study was to determine optimal lipid concentration range for lyophilization of sterically stabilized phospholipid nanomicelles (SSM) and the freeze drying feasibility of self-associated therapeutic peptide-SSM assemblies. SSM at 5-20 mM 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy-poly(ethylene glycol 2000) (DSPE-PEG(2000)) were analyzed for particle size and viscosity before and after freeze drying which showed no significant changes (p > 0.05). However, a steep increase in viscosity was seen for SSM above 15 MM phospholipid implying micelle-micelle interaction. Greater shrinkage of lyophilized cakes was observed below 10 mM phospholipid while they were more fibrous above 15 mM. Therefore, 10-15 mM DSPE-PEG(2000) was chosen as the optimal phospholipid concentration for lyophilized SSM. When vasoactive intestinal peptide (VIP), glucagon-like peptide 1 (GLP-1) or gastric inhibitory peptide (GIP) (each, 67 mu M) was added to SSM (10 mM), formulations showed no significant change in particle size, peptide fluorescence and peptide alpha-helicity before and after lyophilization. In conclusion, we found that peptide drug-SSM interactions are conserved during lyophilization. Published by Elsevier B.V.
|Uncontrolled Keywords:||nanomedicine; nanobiotechnology; drug delivery; nanocarrier; DSPE-PEG(2000); VIP; GLP-1; GIP|
|Subjects:||Biomedical Science > Nanobiotechnology|
Biomedical Science > Nanomedicine
|Deposited By:||Farnush Anwar|
|Deposited On:||07 Jan 2009 17:12|
|Last Modified:||07 Jan 2009 17:12|
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