Ganta, Chanran and Shi, Aibin and Battina, Srinivas K. and Pyle, Marla and Rana, Sandeep and Hua, Duy H. and Tamura, Masaaki and Troyer, Deryl (2008) Combination of nanogel polyethylene glycol-polyethylenimine and 6(hydroxymethyl)-1,4-anthracenedione as an anticancer nanomedicine. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 8 (5). pp. 2334-2340.
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Polyethylene glycol-polyethylenimine (PEG-PEI) nanogels have been used to deliver nucleic acids and oligonucleotides into cells. First, we synthesized PEG-PEI nanogels with methylene proton ratios (CH2O:CH2N) in PEG-PEI ranging from similar to 6.8:1 to 4:1 and less, as shown by H-1 NMR spectra. We first synthesized various nanogels with varying ratios of CH2O:CH2N (methylene proton) in PEG-PEI as shown by H-1 NMR spectra and tested their cytotoxicity using a rodent pancreatic adenocarcinoma cell line (Pan 02). We showed that the nanogel PEG-PEI with methylene proton ratio of 4:1 was strongly cytotoxic to Pan 02 cells in vitro, while the nanogel with the methylene proton ratio of 6.81 was not toxic. We incorporated a novel anti-cancer drug, 6-(hydroxymethyl)-1,4-anthracenedione (AQ) analogue (AQ10) into nontoxic nanogel PEG-PEI and tested the effect of AQ10 loaded nanogel PEG-PEI (AQ10-nanogel PEG-PEI) and AQ10 dissolved in DMSO on Pan 02 cell growth. The size of this AQ10-nanogel PEG-PEI was characterized using atomic force microscopy (AFM). Our studies showed that the AQ10-nanogel PEG-PEI is readily taken up by Pan 02 cells. Growth attenuation of Pan 02 cells treated with AQ10-nanogel PEG-PEI was three to four times that of cells treated with AQ10 dissolved in DMSO. These results suggest that PEG-PEI, usually used to deliver nucleic acids into cells, can also be used to deliver an insoluble small molecule anticancer drug, AQ10.
|Uncontrolled Keywords:||nanogel PEG-PEI; AQ10; Pan 02 cells|
|Subjects:||Biomedical Science > Nanotechnology for human health|
Biomedical Science > Nanomedicine
|Deposited By:||Farnush Anwar|
|Deposited On:||09 Jan 2009 11:43|
|Last Modified:||09 Jan 2009 11:43|
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