Nano Archive

Combination of nanogel polyethylene glycol-polyethylenimine and 6(hydroxymethyl)-1,4-anthracenedione as an anticancer nanomedicine

Ganta, Chanran and Shi, Aibin and Battina, Srinivas K. and Pyle, Marla and Rana, Sandeep and Hua, Duy H. and Tamura, Masaaki and Troyer, Deryl (2008) Combination of nanogel polyethylene glycol-polyethylenimine and 6(hydroxymethyl)-1,4-anthracenedione as an anticancer nanomedicine. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 8 (5). pp. 2334-2340.

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Official URL: http://www.ingentaconnect.com/asp/jnn/2008/0000000...

Abstract

Polyethylene glycol-polyethylenimine (PEG-PEI) nanogels have been used to deliver nucleic acids and oligonucleotides into cells. First, we synthesized PEG-PEI nanogels with methylene proton ratios (CH2O:CH2N) in PEG-PEI ranging from similar to 6.8:1 to 4:1 and less, as shown by H-1 NMR spectra. We first synthesized various nanogels with varying ratios of CH2O:CH2N (methylene proton) in PEG-PEI as shown by H-1 NMR spectra and tested their cytotoxicity using a rodent pancreatic adenocarcinoma cell line (Pan 02). We showed that the nanogel PEG-PEI with methylene proton ratio of 4:1 was strongly cytotoxic to Pan 02 cells in vitro, while the nanogel with the methylene proton ratio of 6.81 was not toxic. We incorporated a novel anti-cancer drug, 6-(hydroxymethyl)-1,4-anthracenedione (AQ) analogue (AQ10) into nontoxic nanogel PEG-PEI and tested the effect of AQ10 loaded nanogel PEG-PEI (AQ10-nanogel PEG-PEI) and AQ10 dissolved in DMSO on Pan 02 cell growth. The size of this AQ10-nanogel PEG-PEI was characterized using atomic force microscopy (AFM). Our studies showed that the AQ10-nanogel PEG-PEI is readily taken up by Pan 02 cells. Growth attenuation of Pan 02 cells treated with AQ10-nanogel PEG-PEI was three to four times that of cells treated with AQ10 dissolved in DMSO. These results suggest that PEG-PEI, usually used to deliver nucleic acids into cells, can also be used to deliver an insoluble small molecule anticancer drug, AQ10.

Item Type:Article
Uncontrolled Keywords:nanogel PEG-PEI; AQ10; Pan 02 cells
Subjects:Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:2702
Deposited By:Farnush Anwar
Deposited On:09 Jan 2009 11:43
Last Modified:09 Jan 2009 11:43

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