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Bicompartmental device for dynamic cell coculture: Design, realisation and preliminary results

Ciofani, Gianni and Migliore, Antonio and Raffa, Vittoria and Menciassi, Arianna and Dario, Paolo (2008) Bicompartmental device for dynamic cell coculture: Design, realisation and preliminary results. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 105 (5). pp. 536-544.

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Official URL: http://dx.doi.org/10.1263/jbb.105.536

Abstract

Low-shear perfusion and compartmental systems are very important conceptual designs for the realization of high-density cell cultures and cocultures, thus allowing very realistic models and therefore reliable results. In this work, we combines low-shear perfusion and multi-compartments in one device. We also describe the design, fabrication and validation of a bicompartmental, low-perfusion culture chamber specifically designed for applications in various fields, such as tissue engineering, pharmacology, molecular biology, pharmacokinetics, nanomedicine, and drug delivery testing. The device properties in terms of flow features and metabolite exchange capabilities are fully characterized. In vitro tests performed on cell lines confirm the effectiveness of the device as an experimental tool for measurements in dynamic and multiple-cell cultures and cocultures.

Item Type:Article
Uncontrolled Keywords:cell culture device; multi-compartmental multiplecell cultures; cellular coculture; bioreactor; dynamic culture; tissue engineering
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:2699
Deposited By:Farnush Anwar
Deposited On:09 Jan 2009 11:11
Last Modified:09 Jan 2009 11:11

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