Zheng, Jing and Feng, Wanjuan and Lin, Li and Zhang, Fan and Cheng, Guifang and He, Pingang and Fang, Yuzhi (2007) A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles. BIOSENSORS & BIOELECTRONICS, 23 (3). pp. 341-347.
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An ultrasensitive and highly specific electrochemical aptasensor for detection of thrombin based on gold nanoparticles and thiocyanuric acid is presented. For this proposed aptasensor, aptamerI was immobilized on the magnetic nanoparticles, aptamerII was labeled with gold nanoparticles. The magnetic nanoparticle was used for separation and collection, and gold nanoparticle offered excellent electrochemical signal transduction. Through the specific recognition for thrombin, a sandwich format of magnetic nanoparticle/thrombin/gold nanoparticle was fabricated, and the signal amplification was further implemented by forming network-like thiocyanuric acid/gold nanoparticles. A significant sensitivity enhancement had been obtained, and the detection limit was down to 7.82 aM. The presence of other proteins such as BSA and lysozyme did not affect the detection of thrombin, which indicates a high specificity of thrombin detection could be achieved. This electrochemical aptasensor is expected to have wide applications in protein monitoring and disease diagnosis.
|Uncontrolled Keywords:||aptamers; nanoparticles; thrombin; electrochemical aptasensor; thiocyanuric acid|
|Subjects:||Physical Science > Nano objects|
Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanobiotechnology
Biomedical Science > Nanotechnology for human health
|Deposited By:||Anuj Seth|
|Deposited On:||27 May 2009 14:12|
|Last Modified:||27 May 2009 14:12|
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