Seelig, J. and Leslie, K. and Renn, A. and Kuhn, S. and Jacobsen, V. and van de Corput, M. and Wyman, C. and Sandoghdar, V. (2007) Nanoparticle-induced fluorescence lifetime modification as nanoscopic ruler: Demonstration at the single molecule level. NANO LETTERS, 7 (3). pp. 685-689.
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Official URL: http://dx.doi.org/10.1021/nl0627590
We combine interferometric detection of single gold nanoparticles, single molecule microscopy, and fluorescence lifetime measurement to study the modification of the fluorescence decay rate of an emitter close to a nanoparticle. In our experiment, gold particles with a diameter of 15 nm were attached to single dye molecules via double-stranded DNA of different lengths. Nanoparticle-induced lifetime modification (NPILM) has promise in serving as a nanoscopic ruler for the distance range well beyond 10 nm, which is the upper limit of fluorescence resonant energy transfer (FRET). Furthermore, the simultaneous detection of single nanoparticles and fluorescent molecules presented in this work provides new opportunities for single molecule biophysical studies.
|Subjects:||Physical Science > Nanophysics|
Analytical Science > Metrology and standards in nanotechnology
|Deposited By:||Anuj Seth|
|Deposited On:||09 Jan 2009 16:15|
|Last Modified:||20 Jan 2009 11:42|
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