Nano Archive

Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing

Lin, Chenxiang and Liu, Yan and Yan, Hao (2007) Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing. NANO LETTERS, 7 (2). pp. 507-512.

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Multiplexed and sensitive detection of nucleic acids, proteins, or other molecules from a single solution and a small amount of sample is of great demand in biomarker profiling and disease diagnostics. Here we describe a new concept using combinatorial self-assembly of DNA nanotiles into micrometer-sized two-dimensional arrays that carry nucleic acid probes and barcoded fluorescent dyes to achieve multiplexed detection. We demonstrated the specificity and sensitivity of the arrays by detecting multiple DNA sequences and aptamer binding molecules. This DNA tile-array-based sensor platform can be constructed through DNA self-assembly. The attachment of different molecular probes can be achieved by simple DNA hybridization so bioconjugation is not necessary for the labeling. Accurate control of the interprobe distances and solution-based binding reactions ensures fast target binding kinetics.

Item Type:Article
Subjects:Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanobiotechnology
ID Code:2603
Deposited By:Anuj Seth
Deposited On:09 Jan 2009 16:37
Last Modified:20 Jan 2009 11:45

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