Lu, Chen-Wen and Hung, Yann and Hsiao, Jong-Kai and Yao, Ming and Chung, Tsai-Hua and Lin, Yu-Shen and Wu, Si-Han and Hsu, Szu-Chun and Liu, Hon-Man and Mou, Chung-Yuan and Yang, Chung-Shi and Huang, Dong-Ming and Chen, Yao-Chang (2007) Bifunctional magnetic silica nanoparticles for highly efficient human stem cell labeling. NANO LETTERS, 7 (1). pp. 149-154.
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Official URL: http://dx.doi.org/10.1021/nl0624263
A superparamagnetic iron oxide (SPIO) nanoparticle is emerging as an ideal probe for noninvasive cell tracking. However, its low intracellular labeling efficiency has limited the potential usage and has evoked great interest in developing new labeling strategies. We have developed fluorescein isothiocyanate (FITC)-incorporated silica-coated core-shell SPIO nanoparticles, SPIO@SiO2(FITC), with diameters of 50 nm, as a bifunctionally magnetic vector that can efficiently label human mesenchymal stem cells (hMSCs), via clathrin- and actin-dependent endocytosis with subsequent intracellular localization in late endosomes/lysosomes. The uptake process displays a time- and dose-dependent behavior. In our system, SPIO@SiO2(FITC) nanoparticles induce sufficient cell MRI contrast at an incubation dosage as low as 0.5 mu g of iron/mL of culture medium with 1.2 x 10(5) hMSCs, and the in vitro detection threshold of cell number is about 1 x 10(4) cells. Furthermore, 1.2 x 10(5) labeled cells can also be MRI-detected in a subcutaneous model in vivo. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes which can still be readily MRI detected. This is the first report that hMSCs can be efficiently labeled with MRI contrast nanoparticles and can be monitored in vitro and in vivo with a clinical 1.5-T MRI imager under low incubation concentration of iron oxide, short incubation time, and low detection cell numbers at the same time.
|Subjects:||Material Science > Nanofabrication processes and tools|
Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
Physical Science > Nanomagnetics
Biomedical Science > Nanotechnology for human health
|Deposited By:||Anuj Seth|
|Deposited On:||12 Jan 2009 09:21|
|Last Modified:||20 Jan 2009 11:51|
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