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Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-Responsive core-shell nanoparticles

Hu, Yuhua and Litwin, Tamara and Nagaraja, Arpun R. and Kwong, Brandon and Katz, Joshua and Watson, Nicki and Irvine, Darrell J. (2007) Cytosolic delivery of membrane-impermeable molecules in dendritic cells using pH-Responsive core-shell nanoparticles. NANO LETTERS, 7 (10). pp. 3056-3064.

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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl071542i

Abstract

Polycations that absorb protons in response to the acidification of endosomes can theoretically disrupt these vesicles via the ``proton sponge'' effect. To exploit this mechanism, we created nanoparticies with a segregated core-shell structure for efficient, noncytotoxic intracellular drug delivery. Cross-linked polymer nanoparticies were synthesized with a pH-responsive core and hydrophilic charged shell designed to disrupt endosomes and mediate drug/cell binding, respectively. By sequestering the relatively hydrophobic pH-responsive core component within a more hydrophilic pH-insensitive shell, nontoxic delivery of small molecules and proteins to the cytosol was achieved in dendritic cells, a key cell type of interest in the context of vaccines and immunotherapy.

Item Type:Article
Subjects:Material Science > Bio materials
Biomedical Science > Nanomedicine
ID Code:2494
Deposited By:Farnush Anwar
Deposited On:07 Jan 2009 12:57
Last Modified:22 Jan 2009 11:19

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