Nativo, Paola and Prior, Ian A. and Brust, Mathias (2008) Uptake and intracellular fate of surface-modified gold nanoparticles. ACS NANO, 2 (8). pp. 1639-1644.
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Official URL: http://dx.doi.org/10.1021/nn800330a
Understanding and controlling the interactions between nanoscale objects and living cells is of great importance for arising diagnostic and therapeutic applications of nanoparticles and for nanotoxicology studies. Here we report a detailed transmission electron microscopy (TEM) study of the uptake of ca. 16 nm surface-modified gold nanoparticles by human fibroblast cells (HeLa cells). It is demonstrated that the well-established enclosomal route of cellular uptake can be bypassed to a significant extent by controlling the uptake mechanism either via the delivery of the nanoparticles by liposomes or by surface modification of the nanoparticles with socalled cell penetrating peptides (CPPs). Successful nuclear targeting is demonstrated using surface modification with a cocktail of CPPs and a peptide acting as a nuclear localization signal (NLS).
|Uncontrolled Keywords:||cellular uptake; gold nanoparticles; liposomes; cell penetrating peptides; nuclear targeting; transmission electron microscopy|
|Subjects:||Physical Science > Nano objects|
Biomedical Science > Nanobiotechnology
|Deposited By:||Anuj Seth|
|Deposited On:||17 Dec 2008 14:27|
|Last Modified:||20 Jan 2009 10:34|
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