Moreau, Christophe J. and Dupuis, Julien P. and Revilloud, Jean and Arumugam, Karthik and Vivaudou, Michel (2008) Coupling ion channels to receptors for biomolecule sensing. NATURE NANOTECHNOLOGY, 3 (10). pp. 620-625.
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Official URL: http://www.nature.com/nnano/journal/v3/n10/abs/nna...
Nanoscale electrical biosensors are promising tools for diagnostics and high-throughput screening systems. The electrical signal allows label-free assays with a high signal-to-noise ratio and fast real-time measurements. The challenge in developing such biosensors lies in functionally connecting a molecule detector to an electrical switch. Advances in this field have relied on synthetic ion-conducting pores(1) and modified ion channels(2-4) that are not yet suitable for biomolecule screening. Here we report the design and characterization of a novel bioelectric-sensing platform engineered by coupling an ion channel, which serves as the electrical probe, to G-protein-coupled receptors (GPCRs), a family of receptors that detect molecules outside the cell. These ion-channel-coupled receptors may potentially detect a wide range of ligands recognized by natural or altered GPCRs(5,6), which are known to be major pharmaceutical targets(7). This could form a unique platform for label-free drug screening(8).
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
Biomedical Science > Nanobiotechnology
Material Science > Nanostructured materials
Biomedical Science > Nanomedicine
|Deposited By:||Anuj Seth|
|Deposited On:||17 Dec 2008 14:35|
|Last Modified:||19 Jan 2009 14:11|
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