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Tip-enhanced Raman spectroscopy can see more: The case of cytochrome C

Yeo, Boon-Siang and Maedler, Stefanie and Schmid, Thomas and Zhang, Weihua and Zenobi, Renato (2008) Tip-enhanced Raman spectroscopy can see more: The case of cytochrome C. JOURNAL OF PHYSICAL CHEMISTRY C, 112 (13). pp. 4867-4873.

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Official URL: http://dx.doi.org/10.1021/jp709799m

Abstract

The heme protein, cytochrome c (Cc) has been studied using tip-enhanced Raman spectroscopy (TERS). By virtue of its sensitivity and superior spatial resolution, TERS detected both the heme and amino acid vibrational bands of Cc using resonance excitation at 532 nm. This is in contrast to conventional surface-enhanced Raman spectroscopy (SERS) where ensemble information is obtained, leading to the strongest Raman bands obscuring the weaker ones; i.e., only the resonantly enhanced heme bands are observed. Matrix-assisted laser desorption/ ionization mass spectrometry supported the interpretation of the Raman data by showing that Cc remains intact on Ag surfaces. This work demonstrates the sensitivity of TERS for analyzing proteins (complete with band assignments) and that a collection of TER spectra gives a more complete description of large biomolecules. The latter is an important advantage usually not found in SERS measurements.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
Material Science > Nanochemistry
Analytical Science > Beam methods
ID Code:2349
Deposited By:Anuj Seth
Deposited On:18 Dec 2008 10:51
Last Modified:18 Dec 2008 10:51

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