Mayer, Kathryn M. and Lee, Seunghyun and Liao, Hongwei and Rostro, Betty C. and Fuentes, Arnaris and Scully, Peter T. and Nehl, Colleen L. and Hafner, Jason H. (2008) A label-free immunoassay based upon localized surface plasmon resonance of gold nanorods. ACS NANO, 2 (4). pp. 687-692.
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Official URL: http://dx.doi.org/10.1021/nn7003734
Robust gold nanorod substrates were fabricated for refractive index sensing based on localized surface plasmon resonance (LSPR). The substrate sensitivity was 170 nm/RIU with a figure of merit of 1.3. To monitor biomolecular interactions, the nanorod surfaces were covered with a self-assembled monolayer and conjugated to antibodies by carbodiimide cross-linking. Interactions with a specific secondary antibody were monitored through shifts in the LSPR spectral extinction peak. The resulting binding rates and equilibrium constant were in good agreement with literature values for an antibody-antigen system. The nanorod LSPR sensors were also shown to be sensitive and specific. These results demonstrate that given a sufficiently stable nanoparticle substrate with a well defined chemical interface, LSPR sensing yields similar results to the surface plasmon resonance technique, yet with much simpler instrumentation.
|Uncontrolled Keywords:||gold nanorod; biosensor; immunoassay; localized surface plasmon resonance; SPR; nanobiotechnology; nanophotonics|
|Subjects:||Physical Science > Nanophysics|
Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
Analytical Science > Beam methods
|Deposited By:||Anuj Seth|
|Deposited On:||18 Dec 2008 11:06|
|Last Modified:||20 Jan 2009 10:38|
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