Nano Archive

A label-free immunoassay based upon localized surface plasmon resonance of gold nanorods

Mayer, Kathryn M. and Lee, Seunghyun and Liao, Hongwei and Rostro, Betty C. and Fuentes, Arnaris and Scully, Peter T. and Nehl, Colleen L. and Hafner, Jason H. (2008) A label-free immunoassay based upon localized surface plasmon resonance of gold nanorods. ACS NANO, 2 (4). pp. 687-692.

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Robust gold nanorod substrates were fabricated for refractive index sensing based on localized surface plasmon resonance (LSPR). The substrate sensitivity was 170 nm/RIU with a figure of merit of 1.3. To monitor biomolecular interactions, the nanorod surfaces were covered with a self-assembled monolayer and conjugated to antibodies by carbodiimide cross-linking. Interactions with a specific secondary antibody were monitored through shifts in the LSPR spectral extinction peak. The resulting binding rates and equilibrium constant were in good agreement with literature values for an antibody-antigen system. The nanorod LSPR sensors were also shown to be sensitive and specific. These results demonstrate that given a sufficiently stable nanoparticle substrate with a well defined chemical interface, LSPR sensing yields similar results to the surface plasmon resonance technique, yet with much simpler instrumentation.

Item Type:Article
Uncontrolled Keywords:gold nanorod; biosensor; immunoassay; localized surface plasmon resonance; SPR; nanobiotechnology; nanophotonics
Subjects:Physical Science > Nanophysics
Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
Analytical Science > Beam methods
ID Code:2346
Deposited By:Anuj Seth
Deposited On:18 Dec 2008 11:06
Last Modified:20 Jan 2009 10:38

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