Nano Archive

Protein-mediated assembly of nanodiamond hydrogels into a biocompatible and biofunctional multilayer nanofilm

Huang, Houjin and Pierstorff, Erik and Osawa, Eiji and Ho, Dean (2008) Protein-mediated assembly of nanodiamond hydrogels into a biocompatible and biofunctional multilayer nanofilm. ACS NANO, 2 (2). pp. 203-212.

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Aqueous dispersible detonation nanodiamonds (NDs) with a diameter of 2-8 nm were assembled into a closely packed ND multilayer nanofilm with positively charged poly-L-lysine via the layer-by-layer deposition technique. The innate biocompatibility of the NDs in both free-floating and thin-film forms was confirmed via cellular gene expression examination by real-time polymerase chain reaction as well as MITT and DNA fragmentation assays. The highly biologically amenable ND nanofilm was successfully integrated with therapeutic molecules, and the functionality of the composite drug-ND material was assessed via interrogation of the suppression of inflammatory cytokine release. Knockdown of lipopolysaccharide-mediated inflammation was observed through the potent attenuation of tumor necrosis factor-alpha, interleukin-6, and inducible nitric oxide synthase levels following ND nanofilm interfacing with RAW 264.7 murine macrophages. Furthermore, basal cytokine secretion levels were assessed to examine innate material biocompability, revealing unchanged cellular inflammatory responses which strongly supported the relevance of the NDs as effective treatment platforms for nanoscale medicine. In addition to the easy preparation, robustness, and fine controllability of the film structures, these hybrid materials possess enormous potential for biomedical applications such as localized drug delivery and anti-inflammatory implant coatings and devices, as demonstrated in vitro in this work.

Item Type:Article
Uncontrolled Keywords:nanomedicine; nanodiamond; bionanotechnology; drug delivery; biointerface; coating
Subjects:Biomedical Science > Nanobiotechnology
Material Science > Nanochemistry
Material Science > Nanostructured materials
Biomedical Science > Nanomedicine
ID Code:2325
Deposited By:Anuj Seth
Deposited On:18 Dec 2008 13:04
Last Modified:19 Jan 2009 14:59

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