Nano Archive

Chemical force microscopy of single live cells

Dague, Etienne and Alsteens, David and Latge, Jean-Paul and Verbelen, Claire and Raze, Dominique and Baulard, Alain R. and Dufrene, Yves F. (2007) Chemical force microscopy of single live cells. NANO LETTERS, 7 (10). pp. 3026-3030.

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Official URL: http://dx.doi.org/10.1021/nl071476k

Abstract

Traditionally, cell surface properties have been difficult to study at the subcellular level, especially on hydrated, live cells. Here, we demonstrate the ability of chemical force microscopy to map the hydrophobicity of single live cells with nanoscale resolution. After validating the technique on reference surfaces with known chemistry, we probe the local hydrophobic character of two medically important microorganisms, Aspergillus fumigatus and Mycobacterium bovis, in relation with function. Applicable to a wide variety of cells, the chemically sensitive imaging method presented here provides new opportunities for studying the nanoscale surface properties of live cells and for understanding their roles in mediating cellular events.

Item Type:Article
Subjects:Analytical Science > Microscopy and probe methods
Biomedical Science > Nanoscale biological processes
Analytical Science > Metrology and standards in nanotechnology
ID Code:2254
Deposited By:Anuj Seth
Deposited On:06 Jan 2009 13:55
Last Modified:19 Jan 2009 16:09

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