Nano Archive

A nanoplasmonic molecular ruler for measuring nuclease activity and DNA footprinting

Liu, Gang L. and Yin, Yadong and Kunchakarra, Siri and Mukherjee, Bipasha and Gerion, Daniele and Jett, Stephen D. and Bear, David G. and Gray, Joe W. and Alivisatos, A. Paul and Lee, Luke P. and Chen, Fanqing Frank (2006) A nanoplasmonic molecular ruler for measuring nuclease activity and DNA footprinting. NATURE NANOTECHNOLOGY, 1 (1). pp. 47-52.

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Interactions between nucleic acids and proteins are essential to genetic information processing. The detection of size changes in nucleic acids is the key to mapping such interactions, and usually requires substrates with fluorescent, electrochemical or radioactive labels(1-3). Recently, methods have been developed to tether DNA to highly water-soluble Au nanoparticles(4-8), and nanoparticle pairs linked by DNA have been used to measure nanoscale distances(9). Here we demonstrate a molecular ruler in which double-stranded DNA is attached to a Au nanoparticle. The change in plasmon resonance wavelength of individual Au - DNA conjugates depends on the length of the DNA and can be measured with subnanometre axial resolution. An average wavelength shift of approximately 1.24 nm is observed per DNA base pair. This system allows for a label-free, quantitative, real-time measurement of nuclease activity and also serves as a new DNA footprinting platform, which can accurately detect and map the specific binding of a protein to DNA.

Item Type:Article
Subjects:Physical Science > Nano objects
Analytical Science > Nanotechnology for sensing and actuating
Biomedical Science > Nanobiotechnology
ID Code:2215
Deposited By:Anuj Seth
Deposited On:06 Jan 2009 16:43
Last Modified:20 Jan 2009 10:56

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