Hupert, Mateusz L. and Guy, W. Jason and Llopis, Shawn D. and Shadpour, Hamed and Rani, Sudheer and Nikitopoulos, Dimitris E. and Soper, Steven A. (2007) Evaluation of micromilled metal mold masters for the replication of microchip electrophoresis devices. MICROFLUIDICS AND NANOFLUIDICS, 3 (1). pp. 1-11. ISSN 1613-4982 (Print) 1613-4990 (Online)
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Official URL: http://www.springerlink.com/content/b26186631385p3...
High-precision micromilling was assessed as a tool for the rapid fabrication of mold masters for replicating microchip devices in thermoplastics. As an example, microchip electrophoresis devices were hot embossed in poly(methylmethacrylate) (PMMA) from brass masters fabricated via micromilling. Specifically, sidewall roughness and milling topology limitations were investigated. Numerical simulations were performed to determine the effects of additional volumes present on injection plugs (i.e., shape, size, concentration profiles) due to curvature of the corners produced by micromilling. Elongation of the plug was not dramatic (< 20%) for injection crosses with radii of curvatures to channel width ratios less than 0.5. Use of stronger pinching potentials, as compared to sharp-corner injectors, were necessary in order to obtain short sample plugs. The sidewalls of the polymer microstructures were characterized by a maximum average roughness of 115 nm and mean peak height of 290 nm. Sidewall roughness had insignificant effects on the bulk EOF as it was statistically the same for PMMA microchannels with different aspect ratios compared to LiGA-prepared devices with a value of ca. 3.7 x 10(-4) cm(2)/(V S). PMMA microchip electrophoresis devices were used for the separation of pUC19 Sau3AI double-stranded DNA. The plate numbers achieved in the micromilled-based chips exceeded I million/m and were comparable to the plate numbers obtained for the LiGA-prepared devices of similar geometry.
|Uncontrolled Keywords:||micromilling; hot-embossing; microchip electrophoresis; polymer microfluidics|
|Subjects:||Engineering > Nanotechnology applications in mechanical engineering|
|Deposited By:||M T V|
|Deposited On:||17 Dec 2008 15:31|
|Last Modified:||17 Dec 2008 15:31|
Repository Staff Only: item control page