Nano Archive

Fluorescent conjugated polymer-stabilized gold nanoparticles for sensitive and selective detection of cysteine

Shang, Li and Qin, Chuanjiang and Wang, Tie and Wang, Ming and Wang, Lixiang and Dong, Shaojun (2007) Fluorescent conjugated polymer-stabilized gold nanoparticles for sensitive and selective detection of cysteine. JOURNAL OF PHYSICAL CHEMISTRY C, 111 (36). pp. 13414-13417. ISSN Print Edition ISSN: 1932-7447; Web Edition ISSN: 1932-7455

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Official URL: http://pubs.acs.org/doi/abs/10.1021/jp073913p

Abstract

We report a new fluorescent detection method for cysteine based on one-step prepared fluorescent conjugated polymer-stabilized gold nanoparticles. The as-prepared fluorescent conjugated polymer-stabilized gold nanoparticles fluoresce weakly due to the fluorescence resonance energy transfer between the fluorophore and the gold nanoparticles. Upon the addition of cysteine, a thiol-containing amino acid, the fluorescence of the colloidal solution increases significantly, indicating that cysteine can modulate the energy transfer between fluorophore and gold. This phenomenon then allows for sensitive detection of cysteine with a limit of detection (LOD) of 25 nM. The linear range of determination of cysteine is from 5 x 10(-8) to 4 x 10(-6) M. None of the other amino acids found in proteins interferes with the determination. Moreover, due to the excellent protecting ability of the fluorescent conjugated polymers, the synthesis of metal nanoparticles and modifying with fluorophores can be accomplished within one step, which makes our method much simpler than conventional methods. We also expect that it will be possible to detect other biologically important analytes based on the fluorescent conjugated polymer-stabilized metal nanoparticles.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:2194
Deposited By:M T V
Deposited On:22 Dec 2008 15:21
Last Modified:22 Dec 2008 15:21

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